TAU Kit
Simoa® Total Tau Kits
The Simoa® Tau Kit is a powerful laboratory tool that precisely measures Tau protein levels in the blood or cerebrospinal fluid (CSF) samples. This kit is designed for use with Quanterix’s Simoa® platform, a digital immunoassay technology that offers ultrasensitivity and precision in biomarker detection.
Quanterix’s Tau Kit
The Simoa® Human Total Tau assay uses a combination of monoclonal antibodies that target the epitope in the N-terminal to mid-domain (R1) of the molecule, designed to recognize all six known splice variants.
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Benefits of using the Quanterix Simoa® Tau Kit
- Ultrasensitive and specific detection of tau protein, allowing for accurate detection and monitoring of neurodegenerative diseases in research applications
- Ability to screen potential drug candidates for their ability to modulate tau protein levels, aiding in the development of new treatments
- Compatible with both serum and CSF samples, providing researchers with a versatile tool for the investigation of tau protein
Tau Protein: Clinical Relevance
Tau is a microtubule-stabilizing protein primarily localized in central nervous system neurons but also expressed at low levels in astrocytes and oligodendrocytes. Tau consists of six isoforms in the human brain, with molecular weights of 48,000 to 67,000 daltons depending on the isoform.
Tau elevation is observed in the CSF of patients with neurodegenerative disease and head injuries, suggesting its extracellular release during neuronal damage and itsa role as a biomarker with specificity for brain injury. Potential movement of elevated CSF tau across the blood-brain barrier presents a possibility that tau measurements in blood could provide a convenient peripheral window into brain/CSF status.
Studies of tau in serum and plasma have been hampered by its low abundance (typically low pg/mL). There are relatively few reports characterizing the appearance of tau in blood or evaluating the usefulness of this biomarker for brain injury assessment. Recent reports using digital immunoassay technology show elevated peripheral tau associated with hypoxic brain injury, concussed hockey players, and repetitive minimal head injury in Olympic boxing.
Applications and Intended Use
The Quanterix Simoa® Tau Kit has been used as a key biomarker assayfor use in discovery and clinical research applications.
The Quanterix Simoa® Tau Kit is a tool for clinical and diagnostic researchers working to advance the understanding and treatment of neurodegenerative diseases and brain injury with a focus on tau protein. Its ultrasensitivity and precision make it a valuable component of any laboratory investigating the role of tau protein in neurological conditions.
- Research on Neurodegenerative Diseases:The Simoa® Tau Kit is a valuable tool for researchers studying neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s. By measuring tau protein levels in the blood, researchers can gain insights into the role of tau protein in the disease process and develop new diagnostics and neuro-prognostics for these debilitating diseases.
- Drug Discovery:Researchers can use the Simoa® Tau Kit to screen potential drug candidates for their ability to modulate tau protein levels. This can help identify promising drug candidates for further development and testing.
- Biomarker Discovery:Tau protein levels in CSF and blood are potential biomarkers for neurodegenerative diseases. Researchers can use the Simoa® Tau Kit to validate these biomarkers, providing researchers with a reliable and accurate tool for biomarker discovery.
Compatible Instruments
The Simoa® Tau Kit is designed for use on the Quanterix HD-X and SR-X instruments.
Assay Performance and Validation Data
Simoa® Tau Kit for SR-X
- Lower Limit of Quantification (LLOQ): 0.0491 pg/mL, pooled CV 9%, mean recovery 95%
- Limit of Detection (LOD):0.0146 pg/mL, range 0.0061-0.0278 pg/mL
- Dynamic range (serum and plasma): 0- ~400 pg/mL
- Dynamic range (CSF): 0- ~1000 pg/mL
- Tests per kit: 96
Simoa® Tau Kit for HD-1/HD-X
- Lower Limit of Quantification (LLOQ): 0.062 pg/mL
- Limit of Detection (LOD): 0.019 pg/mL, range 0.005–0.039 pg/mL
- Dynamic range (serum and plasma): 0–360 pg/mL
- Dynamic range (CSF): 0- ~1000 pg/mL
- Tests per kit: 96