Inhibition Of IFNα Secretion In Cells From Patients With Juvenile Dermatomyositis Under TBK1 Inhibitor Treatment Revealed By Single-molecular Assay Technology
RHEUMATOLOGY (OXFORD, ENGLAND)
Gitiaux C, Bondet V, Bekaddour N, Nusbaum P, Hubas A, Melki I, Bodemer C, Quartier P, Desguerre I, Crow YJ, Herbeuval JP, Duffy D, Bader Meunier B and Rodero MP
Rheumatology (Oxford). 2019 Oct 30. pii: kez508.
Rheumatology Key Message
- Detection of IFNαs proteins secreted by cells from JDM patients opens new perspective for drug discovery.
DEAR EDITOR, A type 1 IFN gene signature has been previously demonstrated in the peripheral blood and muscle of patients with JDM, correlating with disease activity scores. However, direct measurement of IFN alpha (IFNα) protein in samples from patients has remained a challenge until recently. We addressed this limitation by optimizing an ultrasensitive single-molecule array (Simoa) digital ELISA utilizing high affinity autoantibodies isolated from APECED patients that recognize all human interferon-α species . Using this technology, we were able to detect and quantify…