Best practices and guidelines to move your manual assay to your favorite autostainer (Leica BOND RX, Roche DISCOVERY ULTRA). We will review Opal Manual workflow at a high level and then delve into details to convert this to an automated workflow using an autostainer. Tips and tricks will be included.
Learning objectives:
- The steps to automating your manual workflow
- Guidelines for determining optimal Opal dye dilutions
- Pitfalls to avoid during development of your automated assay
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