Opal 3-Plex Detection Kit

The Opal 3-Plex Detection Kit contains:

3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
10X Spectral DAPI
DMSO
1X Plus Automation Amplification Diluent
1X Antibody Diluent/Block
Opal Anti-Ms + Rb HRP
Users should source primary antibodies and other workflow reagents separately. The Opal 3-Plex Detection Kit was optimized for use on the Leica Biosystems BOND RX and configured and validated for imaging on the Vectra® and Mantra™ systems. Kits contain enough reagents to stain up to 50-slides at the recommended 1:150 dilution ratio.

Opal will enable you to:

Measure up to six tissue biomarkers at once
Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
Retain spatial cellular context that is lost in flow cytometry
Confirm single cell co-expression for many biomarkers in one tissue section
Get more information while conserving precious tissue.
Automating Opal staining brings the benefit of:

Quality, consistency and reproducibility with every sample
Time-savings versus laborious manual process
Ease-of-use with a walk-away, high-throughput protocol