Opal 3-Plex Anti-Rb Manual Detection Kit

The Opal 3-Plex Anti-Rb Manual Detection Kit is an immunohistochemistry detection kit that enables multiplex imaging of mouse tissue whilst retaining spatial context. It has been optimized for use on mouse FFPE tissue and configured and validated for imaging on the Vectra®, Vectra® Polaris™ and Mantra™ systems. The Opal 3-Plex Anti-Rb Manual Detection Kit contains:

3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
10X Spectral DAPI
DMSO
1X Plus Manual Amplification Diluent
1X Antibody Diluent/Block
Opal Anti-Rb HRP
1X Opal Anti-Rb HRP Diluent
AR6 Buffer
Users should source primary antibodies and other workflow reagents separately. The Opal 3-Plex Anti-Rb Manual Detection Kit has been optimized for use on mouse FFPE tissue and configured and validated for imaging on the Vectra®, Vectra® Polaris™ and Mantra™ systems. These immunohistochemistry kits contain enough reagents to stain up to 50-slides at the recommended 1:100 dilution ratio.

What is Opal?

Opal Multiplex IHC Kits make multiplex methods accessible to researchers who work with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The Opal IHC multiplexing method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

Measure up to six tissue biomarkers at once
Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
Retain spatial cellular context that is lost in flow cytometry
Confirm single cell co-expression for many biomarkers in one tissue section
Get more information while conserving precious tissue.
Automating Opal staining brings the benefit of:

Quality, consistency and reproducibility with every sample
Time-savings versus laborious manual process
Ease-of-use with a walk-away, high-throughput protocol