One Plasma Sample. More Biological Context.
Blood-based biomarkers allow researchers to characterize multiple components of Alzheimer’s disease biology, including amyloid and tau pathology, neurodegeneration and neuroinflammation. ApoE4 adds another important dimension: underlying disease risk.
ApoE functions primarily in lipid transport, with three predominant alleles in humans: ApoE2, ApoE3 and ApoE4. Its effect on amyloid pathology is isoform-dependent, with ApoE4 showing the greatest effect among the three predominant isoforms.
For researchers already measuring neurology biomarkers, ApoE4 proteotyping allows risk information to be evaluated alongside the molecular biomarker profile. The same EDTA plasma sample type used for core Simoa Alzheimer’s disease biomarker measurements can also support ApoE4 proteotype determination without introducing a separate genotyping workflow.
Explore the Simoa Neurology Portfolio
Amyloid: AB40 | AB42 | pTau217 | pTau181
Tau Pathology: pTau205
Neurodegeneration: NfL
Neuroinflammation: GFAP | YKL-40
Research Use Only
The kit contains reagents for two assays -ApoE4 and pan ApoE- that are run simultaneously from the same sample well. Both measurements are required to determine ApoE4 proteotype. The assays are run together but are not a multiplex.
*ApoE proteotype (haplotype) is based on the ratio of ApoE4 to total ApoE, rather than standalone concentrations.
106115
EDTA plasma
Simoa HD-X
ApoE4 + pan ApoE digital immunoassays used for ratio-based proteotype determination
| ApoE4 | Pan ApoE |
| 0.0074 ± 0.0047 ng/mL | 0.0184 ± 0.0115 ng/mL |
| ApoE4 | Pan ApoE |
| 0.0020–0.0200 ng/mL | 0.0040–0.0370 ng/mL |
| ApoE4 | Pan ApoE |
| 0.051 ng/mL | 0.051 ng/mL |
| ApoE4 | Pan ApoE |
| EDTA plasma | EDTA plasma |
| ApoE4 | Pan ApoE |
| 0.04 µL | 0.04 µL |
Understanding What Shapes Disease Trajectory
ApoE4 status can influence when Alzheimer’s disease emerges, how its biology develops, and how patients respond to therapy. Measuring that status alongside fluid biomarkers creates an opportunity to study not only the presence of disease-associated biology, but the factors that may shape its trajectory.
