Multiplex Measurements of Proteins at the Single Molecule Level

Event Date

Course Description

We will describe the use of single molecule arrays (Simoa) for the multiplex detection of proteins at very low concentrations. We will provide an introduction to the Simoa technology--which is capable of provided a 1000-fold improvement in sensitivity to proteins over conventional assays--and describe how it can be used to detect multiple proteins at the single molecule level. The Simoa HD-1 Analyzer that allows multiplex Simoa assays to be fully-automated from samples-in to results-out will also be described. Simoa users will describe how they have used this technology, including development of a 6-plex for inflammatory cytokines and application of the multiplex Simoa homebrew kit.