Publications & Posters
An ultrasensitive assay format for detecting ULK1 inhibition by monitoring the phosphorylation status of Atg13
Analytical Biochemistry | July 4, 2016
This study focuses on the inhibition of the autophagy initiating kinase ULK1 by measuring the both total Atg13 and the phosphorylation of Atg13(pSer318) from control and following compound treatment in either overexpressing or wild type tissue culture samples.
Serum neurofilament light protein as a marker for diffuse axonal injury - results from a case series study
Journal of Neurotrauma | August 19, 2016
Diffuse axonal injury (DAI) is an important cause of morbidity in patients with traumatic brain injury (TBI). There is a lack of a simple and reliable technique to early identify patients with DAI and to prognosticate long-term outcome in this patient group.
Hospital Medicine Clinics | October, 2016
Troponin is an intracellular regulatory protein complex associated with the contractile apparatus of skeletal and cardiac myocytes. Irreversible injury of cardiac myocytes leading to cell death (myocardial necrosis) results in the release of cTnI and cTnT into the circulation. Highly specific…
Nature Communications | December 2016
Interstitial fibrosis plays a key role in the development and progression of heart failure. Here, we show that an enzyme that crosslinks collagen-Lysyl oxidase-like 2 (Loxl2)-is essential for interstitial fibrosis and mechanical dysfunction of pathologically stressed hearts. In mice, cardiac stress…
Neurology | January 6, 2017
Despite the 3.8 million sports-related concussions (SRC) that occur annually in the United States, there are currently no prognostic biomarkers to predict recovery and an athlete’s readiness to return to play (RTP). An objective predictor of recovery time in the acute aftermath of SRC would provide…
Single-Step Imprinting of Femtoliter Microwell Arrays Allows Digital Bioassays with Attomolar Limit of Detection
ACS Applied Materials & Interfaces | March 7, 2017
Bead-based microwell array technology is growing as an ultrasensitive analysis tool as exemplified by the successful commercial applications from Illumina and Quanterix for nucleic acid analysis and ultrasensitive protein measurements, respectively.
Rhodopsin in plasma from patients with diabetic retinopathy – development and validation of digital ELISA by Single Molecule Array (Simoa) technology
Journal of Immunological Methods | April 5, 2017
The aim of this study was to develop and validate a Rhodopsin assay by employing digital ELISA technology, and to investigate whether Rhodopsin concentrations in diabetes patients with DR are elevated compared with diabetes patients without DR.
The Journal of Experimental Medicine | April 18, 2017
Type I interferons (IFNs) are essential mediators of antiviral responses. These cytokines have been implicated in the pathogenesis of autoimmunity, most notably systemic lupus erythematosus (SLE), diabetes mellitus, and dermatomyositis, as well as monogenic type I interferonopathies.
SLAS Technology| May 22, 2017
Where part I of this review focused on the fundamentals of partitioning and digital PCR, part II turns its attention to digital protein and cell assays. Digital enzyme assays measure the kinetics of single proteins with enzymatic activity.
Bioanalysis| June 19, 2017
Evaluation of three immunoassay-based technologies and their biomarker kits, by determining precision, parallelism and detectability of analytes of interest.