The cerebrospinal fluid (CSF) amyloid-β (Aβ42) peptide is an important biomarker for Alzheimer's disease (AD). Variability in measured Aβ42 concentrations at different laboratories may be overcome by standardization and establishing traceability to a reference system. Candidate certified reference…
Publications & Posters
Assessing the commutability of reference material formats for the harmonization of amyloid beta measurements
Clinical Chemistry and Laboratory Medicine | October 23, 2015
Ultrasensitive Detection and Quantification of Toxins for Optimized Diagnosis of Clostridium difficile Infection.
Journal of Clinical Microbiology | February 1, 2016
Recently developed ultrasensitive and quantitative methods for detection of Clostridium difficile toxins provide new tools for diagnosis and, potentially, for management of C. difficile infection (CDI). Compared to methods that detect toxigenic organism, ultrasensitive toxin detection may allow…
Inhibition of DPP4 activity in humans establishes its in vivo role in CXCL10 post-translational modification: prospective placebo-controlled clinical studies
EMBO Molecular Medicine | April 14, 2016
Biochemical experiments, animal models, and observational studies in humans all support a role of dipeptidyl peptidase 4 (DPP4) in the N-terminal truncation of CXCL10, which results in the generation of an antagonist form of the chemokine that limits T-cell and NK cell migration. This study…
An ultrasensitive assay format for detecting ULK1 inhibition by monitoring the phosphorylation status of Atg13
Analytical Biochemistry | July 4, 2016
This study focuses on the inhibition of the autophagy initiating kinase ULK1 by measuring the both total Atg13 and the phosphorylation of Atg13(pSer318) from control and following compound treatment in either overexpressing or wild type tissue culture samples.
Serum neurofilament light protein as a marker for diffuse axonal injury - results from a case series study
Journal of Neurotrauma | August 19, 2016
Diffuse axonal injury (DAI) is an important cause of morbidity in patients with traumatic brain injury (TBI). There is a lack of a simple and reliable technique to early identify patients with DAI and to prognosticate long-term outcome in this patient group.
Hospital Medicine Clinics | October, 2016
Troponin is an intracellular regulatory protein complex associated with the contractile apparatus of skeletal and cardiac myocytes. Irreversible injury of cardiac myocytes leading to cell death (myocardial necrosis) results in the release of cTnI and cTnT into the circulation. Highly specific…
Nature Communications | December 2016
Interstitial fibrosis plays a key role in the development and progression of heart failure. Here, we show that an enzyme that crosslinks collagen-Lysyl oxidase-like 2 (Loxl2)-is essential for interstitial fibrosis and mechanical dysfunction of pathologically stressed hearts. In mice, cardiac stress…
Neurology | January 6, 2017
Despite the 3.8 million sports-related concussions (SRC) that occur annually in the United States, there are currently no prognostic biomarkers to predict recovery and an athlete’s readiness to return to play (RTP). An objective predictor of recovery time in the acute aftermath of SRC would provide…
Single-Step Imprinting of Femtoliter Microwell Arrays Allows Digital Bioassays with Attomolar Limit of Detection
ACS Applied Materials & Interfaces | March 7, 2017
Bead-based microwell array technology is growing as an ultrasensitive analysis tool as exemplified by the successful commercial applications from Illumina and Quanterix for nucleic acid analysis and ultrasensitive protein measurements, respectively.
Rhodopsin in plasma from patients with diabetic retinopathy – development and validation of digital ELISA by Single Molecule Array (Simoa) technology
Journal of Immunological Methods | April 5, 2017
The aim of this study was to develop and validate a Rhodopsin assay by employing digital ELISA technology, and to investigate whether Rhodopsin concentrations in diabetes patients with DR are elevated compared with diabetes patients without DR.