Publications & Posters
Fifth-Generation Digital Immunoassay for Prostate-Specific Antigen by Single Molecule Array Technology
Clinical Chemistry | October 13, 2011
Measurement of prostate-specific antigen (PSA) in prostate cancer patients following radical prostatectomy (RP) has been hindered by the limit of quantification of available assays. We developed an investigational digital immunoassay with a limit of quantification 2 logs lower than current…
Clinical Evaluation of a Novel Method for Measurement of PSA, AccuPSA™, as a Predictor of 5-year Biochemical Recurrence-free Survival Post Radical Prostatectomy: Results of a Pilot Study
BJU International | October 12, 2011
Nadir Ultrasensitive PSA levels has some value for predicting BCR following RD.
Single molecule measurements of tumor necrosis factor a and interleukin–6 in the plasma of patients with Crohn’s disease.
Journal of Immunological Methods | September 30, 2011
We applied a sensitive, single molecule detection technology to measure TNF–a and IL–6 in the plasma of patients with Crohn’s disease (CD), before and after treatment with anti–TNF–a therapy.
Development of (AccuPSA™), a novel digital immunoassay for subfemtomolar measurement of PSA in postradical prostatectomy patients
2011 AACR, Molecular Diagnostics in Cancer Therapeutic Development, Denver, CO
David Wilson1, David Duffy1, David Rissin1, Cheuk Kan1, Todd Campbell1, Stuart Howes1, David Fournier1, Tomasz Piech1, Gail Provuncher1, Purvish Patel1, Evan Ferrell1, Andrew Rivnak1, Jeff Randall1, Linan Song1, David Walt2.1Quanterix Corporation, Cambridge, MA, 2Tufts University, Boston, MA.
2011 American Academy of Neurology Annual Meeting, Honolulu, Hawaii
D. Wilson1, E. Mortberg2, S. Rubertsson2, H. Zetterberg3, K. Blennow3, L. Song1, L. Chang1, G. Provuncher1, P. Patel1, E. Ferrell1, D. Fournier1, C. Kan1, T. Campbell1, A. Rivnak1, B. Pink1, K. Minnehan1, T. Piech1, D. Rissin1, D.
Simultaneous Detection of Single Molecules and Singulated Ensembles of Molecules Enables Immunoassays with Broad Dynamic Range
Analytical Chemistry | February 23, 2011
We report a method for combining the detection of single molecules (digital) and an ensemble of molecules (analog) that is capable of detecting enzyme label from 10−19 M to 10−13 M, for use in high sensitivity enzyme-linked immunosorbent assays (ELISA).
Detection of prostate specific antigen (PSA) in the serum of radical prostatectomy patients at femtogram per milliliter levels using digital ELISA (AccuPSA™) based on single molecule arrays (SiMoA)
2010 AACC Meeting, Los Angeles, CA
D.C. Duffy1, D. M. Rissin1, C. W. Kan1, T. G. Campbell1, S. C. Howes1, D. R. Fournier1, L. Song1, T. Piech1, P. P. Patel1, L. Chang1, A. J. Rivnak1, E. P. Ferrell1, J. D. Randall1, G. K. Provuncher1, D. R. Walt2. 1Quanterix Corporation, Cambridge, MA, 2Tufts University, Medford, MA.
Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
Nature Biotechnology | May 23, 2010
The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or…
Single Molecule Enzyme Detection and Application to Immunoassay: Implications for Personalized Medicine
2010 ISE International Conference on Enzymes, Crete, Greece
Okrongly, D. Quanterix Corporation, Cambridge, MA
2010 Oak Ridge Conference, San Jose, CA
Rissin DM1, Kan CW1, Campbell TG1, Howes SC1, Fournier DR1, Song L1, Piech T1, Patel PP1, Chang L1, Rivnak AJ1, Ferrell EP1, Randall JD1, Provuncher GK1, Walt DR2, Duffy DC1. 1Quanterix Corporation, Cambridge, MA, and 2Tufts University, Medford, MA.