Publications & Posters
SLAS Technology| May 22, 2017
Where part I of this review focused on the fundamentals of partitioning and digital PCR, part II turns its attention to digital protein and cell assays. Digital enzyme assays measure the kinetics of single proteins with enzymatic activity.
Bioanalysis| June 19, 2017
Evaluation of three immunoassay-based technologies and their biomarker kits, by determining precision, parallelism and detectability of analytes of interest.
Simoa Human Neurology 3-Plex A (N3PA) Immunoassay Measures Amyloid beta1-42, Amyloid beta1-40 and Tau in Blood and CSF Samples Simultaneously
Here we report an automatic multiplexing assay of total Tau, Amyloid beta 1-42 and Amyloid beta 1-40. This fully automated digital immunoassay can measure above 3 biomarkers in serum, plasma and CSF in both healthy and diseased individuals.
Journal of Neurotrauma | August 18, 2017
American football athletes are routinely exposed to sub-concussive impacts over the course of the season. This study sought to examine the effect of a season of American football on plasma tau, a potential marker of axonal damage.
Journal of the American Chemical Society | March 5, 2008
Individual enzyme molecules have been observed to possess discrete and different turnover rates due to the presence of long-lived activity states. If the results reported here can be generalized to other enzymes, then the stochastic nature of individual enzyme molecule kinetics should have a…
Development of (AccuPSA™), a novel digital immunoassay for subfemtomolar measurement of PSA in postradical prostatectomy patients
2011 AACR, Molecular Diagnostics in Cancer Therapeutic Development, Denver, CO
David Wilson1, David Duffy1, David Rissin1, Cheuk Kan1, Todd Campbell1, Stuart Howes1, David Fournier1, Tomasz Piech1, Gail Provuncher1, Purvish Patel1, Evan Ferrell1, Andrew Rivnak1, Jeff Randall1, Linan Song1, David Walt2.1Quanterix Corporation, Cambridge, MA, 2Tufts University, Boston, MA.
Journal of Immunological Methods | April 30, 2012
We have developed a highly sensitive immunoassay—called digital ELISA—that is based on the detection of single enzyme-linked immunocomplexes on beads that are sealed in arrays of femtoliter wells. Digital ELISA was designed to be highly efficient in the capturing of target proteins, labeling of…
Lab on a Chip | April 29, 2013
We have developed a method that enables the multiplexed detection of proteins based on counting single molecules. The simultaneous, specific, and sensitive measurement of several proteins using multiplexed digital ELISA could enable more reliable diagnoses of disease.
JAMA Neurology | March 13, 2014
Determining whether sports-related concussion is associated with elevated levels of blood biochemical markers of injury to the central nervous system and to assess whether plasma levels of these biomarkers predict return to play in professional ice hockey players with sports-related concussion
Clinical Chemistry and Laboratory Medicine | October 22, 2014
Immunoassays measuring cardiac troponins I or T have become firmly established as critical tools for diagnosing acute myocardial infarction.