Publications & Posters
Using antigen-antibody binding kinetic parameters to understand single-molecule array immunoassay performance
Analytical Chemistry | October 25, 2016
This paper provides insights into the performance of single-molecule array (Simoa) immunoassay by examining the effects of various capture and detector antibody-antigen binding kinetic parameters.
Alzheimer's and Dementia | December 19, 2016
•Compared with controls, plasma total tau (t-tau) ≥3.56 was 100% specific to former NFL players. •Greater repetitive head impact exposure predicts higher later-life plasma t-tau. •Further study on plasma t-tau as a screening biomarker for chronic traumatic encephalopathy is warranted.
Lab on a Chip | February 9, 2017
Thomas H. Linz, W. Hampton Henleya and J. Michael RamseyLab Chip DOI: 10.1039/C6LC01415A
Single-Step Imprinting of Femtoliter Microwell Arrays Allows Digital Bioassays with Attomolar Limit of Detection
ACS Applied Materials & Interfaces | March 7, 2017
Bead-based microwell array technology is growing as an ultrasensitive analysis tool as exemplified by the successful commercial applications from Illumina and Quanterix for nucleic acid analysis and ultrasensitive protein measurements, respectively.
Rhodopsin in plasma from patients with diabetic retinopathy – development and validation of digital ELISA by Single Molecule Array (Simoa) technology
Journal of Immunological Methods | April 5, 2017
The aim of this study was to develop and validate a Rhodopsin assay by employing digital ELISA technology, and to investigate whether Rhodopsin concentrations in diabetes patients with DR are elevated compared with diabetes patients without DR.
The Journal of Experimental Medicine | April 18, 2017
Type I interferons (IFNs) are essential mediators of antiviral responses. These cytokines have been implicated in the pathogenesis of autoimmunity, most notably systemic lupus erythematosus (SLE), diabetes mellitus, and dermatomyositis, as well as monogenic type I interferonopathies.
SLAS Technology| May 22, 2017
Where part I of this review focused on the fundamentals of partitioning and digital PCR, part II turns its attention to digital protein and cell assays. Digital enzyme assays measure the kinetics of single proteins with enzymatic activity.
Bioanalysis| June 19, 2017
Evaluation of three immunoassay-based technologies and their biomarker kits, by determining precision, parallelism and detectability of analytes of interest.
Simoa Human Neurology 3-Plex A (N3PA) Immunoassay Measures Amyloid beta1-42, Amyloid beta1-40 and Tau in Blood and CSF Samples Simultaneously
Here we report an automatic multiplexing assay of total Tau, Amyloid beta 1-42 and Amyloid beta 1-40. This fully automated digital immunoassay can measure above 3 biomarkers in serum, plasma and CSF in both healthy and diseased individuals.
Journal of Neurotrauma | August 18, 2017
American football athletes are routinely exposed to sub-concussive impacts over the course of the season. This study sought to examine the effect of a season of American football on plasma tau, a potential marker of axonal damage.