Okrongly, D. Quanterix Corporation, Cambridge, MA
Publications & Posters
Single Molecule Enzyme Detection and Application to Immunoassay: Implications for Personalized Medicine
2010 ISE International Conference on Enzymes, Crete, Greece
2010 Oak Ridge Conference, San Jose, CA
Rissin DM1, Kan CW1, Campbell TG1, Howes SC1, Fournier DR1, Song L1, Piech T1, Patel PP1, Chang L1, Rivnak AJ1, Ferrell EP1, Randall JD1, Provuncher GK1, Walt DR2, Duffy DC1. 1Quanterix Corporation, Cambridge, MA, and 2Tufts University, Medford, MA.
Detection of Single–Molecule DNA Hybridization Using Enzymatic Amplification in an Array of Femtoliter–Sized Reaction Vessels
Journal of the American Chemical Society | September 3, 2008
Improving the sensitivity of DNA biosensors is extremely important in clinical diagnostics, gene therapy, and a variety of other biomedical studies. In this regard, we have developed a highly sensitive single molecule DNA assay platform with a 1fM experimental detection limit using enzymatic…
Journal of the American Chemical Society | March 5, 2008
Individual enzyme molecules have been observed to possess discrete and different turnover rates due to the presence of long-lived activity states. If the results reported here can be generalized to other enzymes, then the stochastic nature of individual enzyme molecule kinetics should have a…
Proceedings of the National Academy of Sciences | September 7, 2007
Inhibition kinetics of single-β-galactosidase molecules with the slow-binding inhibitor D-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy. We…
Digital Readout of Target Binding with Attomole Detection Limits via Enzyme Amplification in Femtoliter Arrays
Journal of the American Chemical Society | April 20, 2006
In this communication, single molecules of β-galactosidase were captured on a 1 mm femtoliter array using biotin−streptavidin binding.
Digital Concentration Readout of Single Enzyme Molecules Using Femtoliter Arrays and Poisson Statistics
Nano Letters| February 21, 2006
Methods for accurately quantifying the concentration of a particular analyte in solution are all based on ensemble responses in which many analyte molecules give rise to the measured signal.