Publications & Posters
Development of (AccuPSA™), a novel digital immunoassay for subfemtomolar measurement of PSA in postradical prostatectomy patients
2011 AACR, Molecular Diagnostics in Cancer Therapeutic Development, Denver, CO
David Wilson1, David Duffy1, David Rissin1, Cheuk Kan1, Todd Campbell1, Stuart Howes1, David Fournier1, Tomasz Piech1, Gail Provuncher1, Purvish Patel1, Evan Ferrell1, Andrew Rivnak1, Jeff Randall1, Linan Song1, David Walt2.1Quanterix Corporation, Cambridge, MA, 2Tufts University, Boston, MA.
2011 American Academy of Neurology Annual Meeting, Honolulu, Hawaii
D. Wilson1, E. Mortberg2, S. Rubertsson2, H. Zetterberg3, K. Blennow3, L. Song1, L. Chang1, G. Provuncher1, P. Patel1, E. Ferrell1, D. Fournier1, C. Kan1, T. Campbell1, A. Rivnak1, B. Pink1, K. Minnehan1, T. Piech1, D. Rissin1, D.
Simultaneous Detection of Single Molecules and Singulated Ensembles of Molecules Enables Immunoassays with Broad Dynamic Range
Analytical Chemistry | February 23, 2011
We report a method for combining the detection of single molecules (digital) and an ensemble of molecules (analog) that is capable of detecting enzyme label from 10−19 M to 10−13 M, for use in high sensitivity enzyme-linked immunosorbent assays (ELISA).
Detection of prostate specific antigen (PSA) in the serum of radical prostatectomy patients at femtogram per milliliter levels using digital ELISA (AccuPSA™) based on single molecule arrays (SiMoA)
2010 AACC Meeting, Los Angeles, CA
D.C. Duffy1, D. M. Rissin1, C. W. Kan1, T. G. Campbell1, S. C. Howes1, D. R. Fournier1, L. Song1, T. Piech1, P. P. Patel1, L. Chang1, A. J. Rivnak1, E. P. Ferrell1, J. D. Randall1, G. K. Provuncher1, D. R. Walt2. 1Quanterix Corporation, Cambridge, MA, 2Tufts University, Medford, MA.
Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
Nature Biotechnology | May 23, 2010
The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or…
Single Molecule Enzyme Detection and Application to Immunoassay: Implications for Personalized Medicine
2010 ISE International Conference on Enzymes, Crete, Greece
Okrongly, D. Quanterix Corporation, Cambridge, MA
2010 Oak Ridge Conference, San Jose, CA
Rissin DM1, Kan CW1, Campbell TG1, Howes SC1, Fournier DR1, Song L1, Piech T1, Patel PP1, Chang L1, Rivnak AJ1, Ferrell EP1, Randall JD1, Provuncher GK1, Walt DR2, Duffy DC1. 1Quanterix Corporation, Cambridge, MA, and 2Tufts University, Medford, MA.
Detection of Single–Molecule DNA Hybridization Using Enzymatic Amplification in an Array of Femtoliter–Sized Reaction Vessels
Journal of the American Chemical Society | September 3, 2008
Improving the sensitivity of DNA biosensors is extremely important in clinical diagnostics, gene therapy, and a variety of other biomedical studies. In this regard, we have developed a highly sensitive single molecule DNA assay platform with a 1fM experimental detection limit using enzymatic…
Journal of the American Chemical Society | March 5, 2008
Individual enzyme molecules have been observed to possess discrete and different turnover rates due to the presence of long-lived activity states. If the results reported here can be generalized to other enzymes, then the stochastic nature of individual enzyme molecule kinetics should have a…
Proceedings of the National Academy of Sciences | September 7, 2007
Inhibition kinetics of single-β-galactosidase molecules with the slow-binding inhibitor D-galactal have been characterized by segregating individual enzyme molecules in an array of 50,000 ultra small reaction containers and observing substrate turnover changes with fluorescence microscopy. We…