Publications & Posters
48 Single Molecule Array Ultra-Sensitive Cytokine Assays in EDTA Plasma Are Robust and Clinically Feasible
American Journal of Clinical Pathology | January 11, 2018
The objective of this study was to clinically validate a new ultra-sensitive immunoassay platform with EDTA plasma patient specimens for eventual translation as a blood-based biomarker in immuno-oncology.
Nature Communications | December 19, 2017
Microbial nucleic acid recognition serves as the major stimulus to an antiviral response, implying a requirement to limit the misrepresentation of self nucleic acids as non-self and the induction of autoinflammation. By systematic screening using a panel of interferon-stimulated genes we identify…
Journal of Immunological Methods | November 06, 2017
Results from the study suggest that sweat patches are a feasible method to collect cytokine data from older adults.
EBioMedicine | November 03, 2017
Study objective was to identify biomarkers with a prognostic capacity of one-year mortality. We employed an observational cohort of HFP (n = 60) followed-up longitudinally during the first year post fracture.
The Journal of Experimental Medicine | April 18, 2017
Type I interferons (IFNs) are essential mediators of antiviral responses. These cytokines have been implicated in the pathogenesis of autoimmunity, most notably systemic lupus erythematosus (SLE), diabetes mellitus, and dermatomyositis, as well as monogenic type I interferonopathies.
Journal of Experimental Medicine | April 18, 2017
Measurement of IFNα attomolar concentrations by digital ELISA will enhance our understanding of IFN biology and potentially improve the diagnosis and stratification of pathologies associated with IFN dysregulation.
Evaluation Of Th2 And Th17 Cytokine Levels In Serum From Asthmatics Using The High Sensitivity Quanterix Simoa HD-1 Platform
American Thoracic Society | May 15, 2016
This study investigated the capability of the Quanterix Simoa HD-1 to measure interleukin (IL)-5 , - 13, and -17A in serum samples to determine if increasing assay sensitivity will allow us to convincingly detect these cytokines at baseline.
Development of an ultra-sensitive Simoa assay to enable GDF11 detection: a comparison across bioanalytical platforms
Bioanalysis | February 26, 2016
Four bioanalytical platforms were evaluated to optimize sensitivity and enable detection of recombinant human GDF11 in biological matrices. Simoa achieved the greatest sensitivity, an improvement of 100-fold over the next sensitive platform.
Immunochemistry for high-throughput screening of human exhaled breath condensate (EBC) media: implementation of automated Quanterix SIMOA instrumentation
Journal of Breath Research| December 11, 2015
Immunochemistry is an important clinical tool for indicating biological pathways leading towards disease. Standard enzyme-linked immunosorbent assays (ELISA) are labor intensive and lack sensitivity at low-level concentrations. Here we report on emerging technology implementing fully automated…
Single molecule array (Simoa) assay with optimal antibody pairs for cytokine detection in human serum samples
Analyst | August 5, 2015
We provide detailed antibody pair information as well as the concentration profiles of ten cytokines in healthy human serum to serve as reference data for further ultrasensitive immunoassay development and future clinical applications.