EUROPEAN SOCIETY FOR CLINICAL VIROLOGY VIRTUAL MEETING ON COVID-19
September 9, 2020
Industry Symposium / 13:15 - 14:05 CEST (7:15 am - 8:05am EDT) / Hall B
High Definition Simoa Ultra-sensitive Cytokine Profiling for COVID-19 Research: Multiplexing with No Compromise on Sample Readability and Robustness
Paula Perin, PhD
Senior Field Application Scientist, Quanterix
Cytokines are critical messengers of innate and adaptive immune responses. A fine- tuned balance of Th1/Th2 cytokines at appropriate levels is essential to clear viral infection with minimal harm to the host. SARS-CoV-2 infection is known to induce hyperinflammation/cytokine storm in a number of patients, which often leads to severe and critical COVID-19 disease. Profiling of early biomarkers to predict severe/critical disease is an essential consideration in a preventive approach. It also allows for better characterization of the longitudinal inflammatory process in response to SARS-CoV-2 infection. Quantification of baseline levels of circulating cytokines enables identification of early rises of cytokines, use of low sample volumes and highly precise and reproducible measurements for more robust statistical data analysis. However, quantification of cytokines in multiplex is critical, considering their pleiotropic, additive, synergistic or antagonistic effects. In this presentation, we will cover how the Simoa planar technology allows quantification of cytokine profiles (10-plex, Th1/Th2 cytokines) at ultra low levels in healthy controls and COVID-19 patient samples, without compromising sensitivity and quality of the data.