Support Across the Full Biomarker Continuum from Discovery to Clinical Implementation
Whole-slide imaging. Full context. Deeper insights.
Akoya PhenoCycler-Fusion 2.0
Designed for Ultra-High Plex Discovery Research
- Simultaneously profile 100+ biomarkers per tissue section
- Ideal for biomarker discovery from single samples to large-scale, population-level analyses
Akoya PhenoImager-HT 2.0
Designed for High-Throughput Translational & Clinical Research
- Process 300+ samples per week for biomarker validation and clinical research applications
- Rapid, reproducible analysis with streamlined workflows optimized for large-scale studies
Spatial Proteomic Services to accelerate your research
Our CLIA-certified laboratory provides a range of research services to meet, scale, and fast-track your biomarker discovery, translational and clinical trial research needs. Leverage our decades of spatial biology and regulatory expertise to advance your programs efficiently and with confidence.
Custom Biomarker & Assay Development
- Custom Biomarker Development
- Multiplex Biomarker Panel Design
- Panel Optimization & Validation
Sample Testing & Biomarker Analysis
- High-Plex Biomarker Discovery
- High-Throughput Clinical Assay Development
- Bioinformatics & Image Analysis
ADC & Precision Medicine Development
- Antibody-drug conjugate (ADC) evaluation
- Immunotherapy assessment
- Target Identification
Bench-to-Market Diagnostic Development
- Clinical Trial Assay (CTA) Development & Validation
- Companion Diagnostic (CDx) Support
- Laboratory-Developed Test (LDT) Services
How We Accelerate Your Program’s Pipeline?
Our PhD- and ASCP-certified team brings decades of experience in multiplex assay development and validation, grounded in regulatory and clinical development standards.
We have supported the development, optimization, and validation of 400+ spatial biomarkers, 130+ multiplex assays across diverse disease indications, 3+ laboratory-developed tests (LDTs), and worked with more than 40 global pharmaceutical companies. Our expertise enables teams to troubleshoot challenges quickly and advance programs with greater speed and confidence.
We have a dedicated Quality Assurance oversight team and maintain GCLP certification, ISO 15189 accreditation through A2LA, CAP/CLIA-accredited pathology integration, and GxP-compliant processes for clinical trial sample testing.
Our workflows are supported by board-certified clinical pathologists ensuring accurate interpretation across both research and clinical applications.
Our dedicated BSL-2 laboratory is designed for high-throughput, high- and low-plex spatial workflows. With multiple Akoya PhenoImager-HT and Akoya PhenoCycler-Fusion instruments we support rapid turnaround times, processing 300+ samples per week while maintaining rigorous quality standards.
Our global infrastructure supports scalable multi-site, longitudinal, and high-sample-volume studies.
As inventors of the Akoya PhenoImager™ technology (formerly Phenoptics™), we bring unmatched expertise in multiplex imaging and cutting-edge spatial technologies. We are uniquely positioned to rapidly design and develop your next-generation spatial biomarker assays for emerging and complex applications.
We offer flexible, fit-for-purpose services to support research-use-only (RUO) projects, clinical trial (CTA), laboratory developed tests (LDT), and companion diagnostics (CDx) applications. Our scientific and operational support is tailored to each program, helping teams scale, maximize resources, and make faster decisions.
Custom Biomarker & Assay Development
Leverage our molecular barcoding expertise to streamline your spatial proteomic assay development. We can barcode customer-selected antibodies and integrate custom targets into existing or de novo multiplex panels.
We can run additional characterization, functional testing, and optimization for clients who need to fast track development of high-performance, reproducible biomarkers and biomarker panel assays.
Custom Antibody Barcoding
Leverage our curated antibody library or submit your own unique antibodies for custom barcoding. Custom barcoded antibodies can be delivered directly to your lab independent or incorporated into custom assay development or sample testing studies.
- Save time, cost, and labor
- Bulk barcoding available
- Intended use on Mouse and Human FFPE tissue types; Book consult for related species tissue types and emerging applications
Custom-Barcoding Workflow
A high-level look at our custom-barcoding workflow from antibody selection to deliverable.
Barcode Selection
Selection based on biomarker expression level and compatibility with off-the-shelf panels or other custom antibodies
Molecular Barcoding Conjugation
SDS-Page gel to confirm barcoding
Functional Testing
(Optional)
- Testing of up to 10 barcoded antibodies on a single positive control slide.
- Testing includes 1 round of staining followed by qualitative and quantitative assessments.
Antibody Optimization
(Optional)
- Optimization of up to 10 barcoded antibodies on a single positive control slide.
- Optimization includes up to 2 rounds of staining followed by qualitative and quantitative assessments.
Deliverables
- Barcoded antibody
- Reporter-fluorophore
- Conjugation report
For projects including functional testing or optimization, additional deliverables include:
- Whole slide QPTIFF images
- Testing or optimization reports
Custom Assay Development
We can design the panel your science demands. Fit-for-purpose custom multiplex spatial proteomics panels tailored to your therapeutic area and study objectives. Our workflows support panel customization, expansion, and optimization, with tiered validation for RUO, CTA, LDT (CAP/CLIA), and CDx applications to enable rapid deployment.
- Save time, cost, and labor
- Reliable multiplex panels
Intended use on Mouse and Human FFPE tissue types; Book consult for related species tissue types and emerging applications
Custom Assay Panel Options
We can expand or swap biomarkers in our off-the-shelf mIF panels, combine biomarkers across panels, or build de novo panels tailored to your research or therapeutic area. We offer end-to-end service from panel design, optimization, and validation with spatial analysis, if incorporated into sample testing study.
General Workflow
- Select Existing Panel to Customize or Full Custom Panel
- Custom Biomarker Barcoding
- Panel Optimization
- Custom panels can be incorporated into sample testing studies for end-to-end workflow
Customize Off-the-Shelf PhenoCode™ Discovery Panels
Immuno-Oncology Panels
Neurobiology Panels
De Novo Custom Panel Development
- Fully customized spatial panel
- Novel biomarkers of interest
- Develop on indication of interest
- Board-certified pathologist review
Sample Testing & Biomarker Analysis
From biomarker discovery to spatial signature clinical implementation. We offer the most advanced and complete solution for multiplex tissue analysis. Leverage our end-to-end sample testing spatial services from study design through data interpretation and regulatory readiness.
End-to-end support includes tissue and slide preparation, histology, spatial analysis, and board-certified pathology review and annotation. Dedicated project management and regulatory support to ensure quality and reduce risk across your development pipeline.
Tissue Sample Testing
Leverage our decades of spatial and translational expertise to generate high-quality spatial biology data from your tissue samples. Submit mounted FFPE tissue sections, TMAs, multi-tissue blocks and select the assay panel that best fits your study. We can run sample testing using our ready-to-use multiplex PhenoCode™ panels, customize an existing panel from our portfolio, or develop a fully custom panel tailored to your research needs.
All tissue samples undergo board-certified pathology review, annotation, and region-of-interest identification before testing.
Capabilities & Advantages
- Unbiased, high-plex single cell biomarker discovery with 100+ biomarkers per tissue
- High-throughput, reproducible translational and clinical workflows, processing 300+ samples per week
- CLIA/GCLP/ISO certified laboratory
- FDA submission and regulatory support
Sample Testing Panel Selection & Workflow
A high-level look at sample testing panel options and workflow from sample submission to data and reporting. Fast-track your insights with our ready-to-use PhenoCode™ panels.
Workflow Overview
- Study consultation
- Panel selection (PhenoCode™ or custom panel)
- Sample submission
- Board-Certified pathology review & annotation
- Panel optimization
- Whole-slide, high-speed spatial imaging
- Image analysis (Single-cell segmentation, phenotyping, spatial analysis)
- Data & reporting; Standard or Advanced data package (deliverables & insights tailored to study objectives)
Immuno-Oncology PhenoCode™ Discovery Panels
Optimized, ready-to-use high-plex biomarker panels:
Key Applications:
- Characterize TME
- Immunotherapy studies (checkpoint inhibitors, resistance mechanisms, immune activation pathways, novel target identification)
- Biomarker discovery
- Patient Stratification
Neurobiology PhenoCode™ Discovery Panels
Optimized, ready-to-use high-plex biomarker panels:
Key Applications:
- Neurodegeneration research (Alzheimer’s disease, Parkinson’s disease, protein aggregates)
- Neuroinflammation studies
- Blood-brain barrier investigations
- Vascular biology
- Glial biology
Custom Panel
Fully customized high-plex and low-plex panels.
- Expand or customize off-the-shelf panels
- Fully custom de novo panel
- Novel biomarkers of interest
- Develop on indication of interest
- Board-certified pathologist review
Built on the technology we pioneered, we can fast-track your program from high-plex biomarker discovery to clinical implementation—combining advanced spatial analysis expertise to identify actionable signatures and rapid panel development to translate them into custom low-plex panels for clinical implementation
Bioinformatics & Image Analysis
If you have a challenging research question or don’t have access to bioinformaticians, we can help. We have a dedicated image analysis team with extensive bioinformatics experience. Using a proprietary imaging analyses pipelines, only available through our services, we can help you answer the most challenging questions.
Send us your QPTIFF images from samples run on an Akoya instrument and our team will work with you to answer your research questions.
- Faster, deeper, reliable data insights
- Proprietary image analysis pipelines
- Standard spatial analysis package
- Advanced spatial analysis or comparison of biological groups package
Bioinformatics Workflow
A high-level look at our image and data analysis only workflow.
Spatial Consultation
Discuss with our informatics team your study, research questions, and timeline to design and identify optimal data analysis strategy
QPTIFF Images
Send us your QPTIFF images for QC and analysis
Data Analysis
Select either Standard or Advanced data analysis package.
Standard analysis includes identification of all major cell types, visualization of spatial distribution, and quantification of abundance.
Book a consult to discuss advanced analysis options.
ADC & Precision Medicine Development
Fast-track your ADC and immunotherapy programs at scale. We provide multiplex spatial antibody-drug conjugate (ADC) and targeted therapeutic development with end-to-end support from panel design and development through sample testing and regulatory readiness.
Antibody-Drug Conjugate Development
Advance and de-risk your program. Leverage our validated ADC biomarker panels and standardized workflows for reliable translational decision making for ADC programs across multiple disease indications, including breast and non-small cell lung cancer.
Accelerator Advantages
- High-sensitivity staining to detect ultra-low expression
- High-resolution imaging with on-board spectral unmixing
- Proprietary membrane specific markers to easily distinguish membrane vs cytoplasmic expression
- Digital DAB for each biomarker for pathologist’s review
- Regulatory-grade data packages
Validated ADC Panels
Leverage or customize our ADC biomarker panels for target quantification and screening, archive-to-fresh bridging studies, on-treatment pharmacodynamic (PD) biopsy analysis, heterogeneity characterization, resistance mechanism mapping, bystander-derived kill-radius evaluation, and more.
Breast Cancer ADC Panel
Panel biomarkers: HER2, Trop2, Ki67, ER/PR, membrane cocktail
Leverage for patient enrichment for HER2-low ADC trials, resistant metastatic biopsies, on-treatment biopsies to show PD
NSCLC ADC Core Panel
Panel biomarkers: B7-H3, HER2, TROP2, c-Met, PanCK, membrane cocktail
Leverage for high c-Met biopsies, mapping EGFR-mut resistance, TME proximity for IO-ADC combinations
NSCLC ADC Evolve Panel
Panel biomarkers: EGFR, CEACAM5, MSLN, HER3, PanCK, membrane cocktail
Leverage for resistance biopsy mapping past-TKI, emerging HER3 programs, mesothelioma/CEA-ADC exploratory screening, spatial PD signatures in combination regimens
Immunotherapy Development
Reveal how cells interact in their native tissue environment to unlock biomarker insights that drive the development of better immunotherapies. Understand why patients respond — or fail to respond to therapy.
Study Examples
- Checkpoint inhibitor studies
- Resistance mechanisms
- Immune activation pathways
- Novel target identification
Bench-to-Market Diagnostic Development
For diagnostics to truly impact patient care, results must be consistent and reproducible regardless of which lab performs the test or who runs the assay.
Our development solutions focus on delivering consistent, reproducible results across laboratories, instruments, and operators. Our approach follows a rigorous, risk-based framework aligned with FDA expectations and ISO 13485 quality standards, helping ensure reliable performance from development through commercialization.
CTA, LDT, CDx Development
Whether you are launching your first assay development program or expanding an established pipeline, the Accelerator Spatial team operates as an extension of your team – collaborative, responsive, and committed to shared success. We provide technical and strategic partnership across the full program lifecycle including project management and milestone support, access to a board-certified pathologist, and regulatory support for FDA submissions.
Together, we can help translate spatial biology innovation into meaningful impact for patients.
- Clinical trial assay development & validation
- Patient testing
- Diagnostic readiness
- Regulatory support
Spatial Clinical Collaborations
Read more about some of our spatial proteomic collaborations and see how spatial proteomics is being leveraged and deployed in clinical research.
AidaBREAST™
Collaboration with PreludeDx lead to development of a novel multi-omic assay on the Akoya PhenoImager-HT 2.0 with Opal Chemistry, becoming the first to predict both locoregional recurrence risk and radiation therapy benefit in early-stage invasive breast cancer
Immunoprint®
A 7-biomaker mIF assay develop and validated by board-certified pathologists demonstrated analytical equivalence to traditional IHC assays in stratifying early-stage melanoma patients and identifying high-risk subgroups.
OncoSignature®
The first-of-its-kind spatial signature companion diagnostic assay to identify patients with ovarian or endometrial cancers for a targeted oncology agent.
Discover More
Akoya PhenoCycler-Fusion 2.0
A discovery platform capable of high-speed, high-plex, whole-slide imaging
Akoya PhenoImager-HT 2.0
A high-throughput, low-plex multispectral imaging platform with onboard spectral unmixing
Interactive Datasets
Explore spatial biology datasets created using ultrahigh-plex advanced spatial proteomics in an interactive data gallery.
Discuss your project, request a quote, or not sure where to start?
Tell us about your study goals and we’ll recommend the best platform and workflow.