Next-Generation PhenoCode Molecular Barcoding Chemistry: Enhanced Reliability and Reproducibility for High-Plex Spatial Profiling
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Poster Background
Reliable and reproducible antibody barcoding is critical for spatial biology workflows on
the Akoya PhenoCycler®-Fusion 2.0 (PCF). We evaluated and optimized a complementary
chemistry to the existing Antibody Conjugation Kit (7000009), culminating in the novel PhenoCode®
Molecular Barcoding Kit (7000020). The new method demonstrated improvements in conjugation
success and reproducibility. This innovative chemistry maintains the immunoglobulin’s
sensitivity, specificity and end-user confidence while retaining the simple workflow and
reduced dependence on end-user’s skill.
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