Create your customized PhenoCode Signature Immune Human Protein Panel
The PhenoCode Signature Panels are validated for the PhenoImager HT 2.0 and PhenoImager Fusion that integrate staining, imaging, and analysis using well-established workflows while providing accuracy and reproducibility.
There are three steps to ordering your PhenoCode Signature Panel that includes a novel checkpoint or immune cell marker into a five-plex base panel.
Order the PhenoCode Signature Immune Human Protein Panel
The five-plex panel includes CD20, CD3e, CD68, and CD8, and PanCK.
Select an additional antibody
Select a validated antibody ready to use with the panel OR use the Antibody Conjugation Kit for PhenoCode Signature with your antibody of choice.
Detect your additional antibody
Include the Opal 520 Reagent Pack in your order to detect the selected antibody.
Get the answer to your question
Our validated antibodies are barcoded and ready to add to the immuno-contexture panel. Choose your antibody from the options on the left to answer your specific question.
- CD163 – Where are the immunosuppressive macrophages?
- CD4 – Where are the helper T cells?
- CD45RO – Where are the memory T cells?
- FoxP3 – Where are the regulatory T cells?
- Granzyme B (GrzB) – Where are the activated immune cells?
- Ki67 – Which cell types are proliferating?
- PD-L1 – Where is immune checkpoint expression?
- PD-1 – Are the T cells exhausted?
- Smooth muscle actin (SMA) – Where are the stromal or smooth muscle cells?
Kit contents
Five primary antibodies
CD20, CD3e, CD68, and CD8, and pan cytokeratin (PanCK)
Five PhenoCode Signature Detectors
Five Opal dyes
Opal 480, Opal 570, Opal 620, Opal 690, and Opal 780 + TSA-DIG
Dimethyl sulfoxide (DMSO)
10X Spectral DAPI
Buffers
1X Plus Automation Amplification Diluent, PhenoCode Signature Diluent, and 1X Antibody Diluent/Block
Blockers
PhenoCode Signature Blocker 1, PhenoCode Signature Blocker 5, N Blocker, J Blocker, and S Blocker
Documents
The following documents are provided to support your research
Related Resources
Explore these resources to learn how PhenoCode Signature Panels accelerate spatial insights and support immunotherapy research.
PhenoCode Signature Panels Brochure
Get an overview of PhenoCode Signature Panels and how they enable comprehensive tumor microenvironment analysis.
Decoding the Science of Response to Cancer Therapy
Learn how spatial phenotyping with PhenoCode Signature Panels reveals biomarkers linked to therapeutic response.
Six-Plex for Success
Discover how multiplexed biomarker analysis enhances immune profiling and accelerates research.
A Comprehensive Approach to Immunotherapy Response Prediction
See how complementary PhenoCode Signature Panels unlock spatial signatures for predicting treatment outcomes.
Predictive Role of Spatial Phenotyping in NSCLC
Explore research on spatial biomarkers and their potential predictive role in lung cancer therapy.
Find the Panel That Answers Your Next Question
Each PhenoCode Signature Panel is curated to reveal unique aspects of the tumor microenvironment—choose the one that fits your research goals.
PhenoCode Signature Activated TIL Status Human Protein Panel
Including biomarkers CD8, CD3e, Ki67, Granzyme B, and PanCK, this panel determines Ki67 positivity in addition to profiling of the TME for activated CD8-positive T lymphocytes.
PhenoCode Signature Immuno-Contexture Status Human Protein Panel
Including biomarkers CD68, CD8, FoxP3, PanCK, and PD-L1, this panel characterizes tumors as hot or cold and therefore likely response to checkpoint inhibitor immunotherapy.
PhenoCode Signature T Cell Status Human Protein Panel
Including biomarkers CD8, CD4, CD20, FoxP3, and PD-1, this panel identifies distinct T-cell subsets and effector functionality status.
Request a consultation or quote
If you would like to learn more about the PhenoCode Signature Immune Profile Human Protein Panel, simply submit the form below and one of our technical specialists will get in touch with you.