Why Choose the Mouse IO PhenoCode Discovery Panel?
Fast, Ready-to-use: Optimized for mouse FFPE tissue on the PhenoCycler-Fusion 2.0 to deliver rapid, reproducible, high-plex imaging
Translational: Designed around human disease-relevant biomarkers, seamlessly transition from mouse to human with our Edison Awards Silver Award-Winning Human IO60 Protein PhenoCode Discovery Panel for continuity across preclinical and clinical neuroscience
Multi-Omics Compatible: Gentle chemistry preserves tissue quality to support additional ‘omics analyses on the same tissue section without limiting plexing
Scalable: Expand and customize the panel by adding additional biomarkers using our PhenoCode 2.0 Molecular Barcoding Kit
Biomarkers Included in the Panel
The Mouse IO Discovery Panel features 23 biomarkers for multiplex immunofluorescence of key immune cell populations in the tumor microenvironment. Tested and optimized for mouse FFPE tissue, the panel provides robust performance across commonly used preclinical sample types. For alternative tissue preparations or preclinical models, our Spatial Accelerator Research Services team provides custom assay development, sample testing, and proof-of-concept studies to assess panel performance for your specific translational needs.
| T Cell Markers | |
|---|---|
| T Cell |
CD3e
|
| Helper T Cell | CD4 |
| Cytotoxic T Cell | CD8 |
| Regulatory T Cell | FOXP3 |
| Activated T Cell | CD44 |
| Naive T Cell | CD45R/B220 |
| B Cell Markers | |
|---|---|
| B Cell |
CD20 |
| Structural, Proliferation and Tumor Markers | |
|---|---|
| Mesenchymal cells, epithelial-mesenchymal transition (EMT) | Vimentin |
| Extracellular Matrix |
Col1A1 |
| Integral Membrane Protein | Caveolin |
| Epithelial Cells, Tumor Marker | PanCK |
| Proliferation Marker | Ki67 |
| Other Cell Type Markers | |
|---|---|
| Endothelial Cells | CD31 |
| Adipocyte Progenitors | CD36 |
| Erythroid Cells | Ter119 |
| Macrophage Markers | |
|---|---|
| Macrophage | F4/80 |
| M2 Macrophage | CD206 |
| Activated Macrophages | CD68 |
| Immune Cell Markers | |
|---|---|
| Peripheral Neutrophils, Monocytes and Granulocytes | Ly6g |
| Multiple Immune Cell Types | FCRγ |
| Nucleated Hematopoietic Cells | CD45 |
| Microglia | Iba1 |
| Myeloid Differentiation | S100A9 |
Explore our database of validated antibodies to streamline adding additional biomarkers.
Key Research Applications:
Tumor microenvironment (TME) characterization: Analyze the spatial organization and abundance of immune populations within the TME, including immune infiltration patterns, immunosuppression niches, and stromal architecture
Immune cell spatial phenotyping: Profile and characterize immune composition and activation state at single-cell resolution within tumors
Translational IO research: Examine mechanisms driving tumor progression and therapeutic response in mouse models that can be compared with human IO spatial findings to improve translational relevance during therapy development
Drug response and biomarker discovery: Evaluate how experimental immunotherapies alter immune composition, proliferation, and tissue architecture to identify potential response biomarkers in preclinical studies
Identify predictive spatial signatures: Link cell-specific protein expression and identify predictive immune signatures associated with treatment response and resistance in preclinical models
FFPE-based archival tissue studies: Leverage stored murine FFPE, including tissue microarrays, from prior preclinical studies to retrospectively analyze spatial immune architecture, tumor biology, and therapeutic response without repeating in vivo experiments
Explore how researchers designed and developed a custom mouse FFPE Akoya PhenoCycler-Fusion Discovery Panel to study the TME in a murine model of melanoma, leading to insights into nanoparticle reprogramming of melanoma.
The panel is available in two configurations:
Mouse IO PhenoCode Discovery Panel
Purchase only the 24-marker Mouse IO Panel, which includes antibodies and reporters. The panel supports up to 25 slides at a 1:200 dilution.
Mouse IO PhenoCode Discovery Starter Pack
The starter pack includes the Mouse IO PhenoCode Discovery Panel plus Akoya PhenoCycler-Fusion consumables for a complete solution to spatially phenotype 24 IO biomarkers. The starter pack supports up to 20 slides.
Discover More
Request a Quote or Speak with an Expert
Whether you’re evaluating the Mouse IO Discovery Panel for your research or requesting pricing, our team can help. Submit the form below and a spatial biology specialist will reach out soon.