Why Choose the Human Metabolism Spike-In Module?
Comprehensive Metabolic Profiling of the TME: Fully compatible with the Human IO60 Discovery Panel, enabling spatial profiling of key metabolic pathways that regulate cellular function, immune activity, and tumor biology at single-cell resolution
Ready-to-Use Workflow: Optimized for the Akoya PhenoCycler-Fusion workflow to enable rapid, reproducible, ultrahigh-plex multiplex immunofluorescence imaging
Multi-Omics Compatible: Gentle chemistry preserves tissue integrity and supports downstream multi-omics analyses from the same tissue section without limiting plexing
Scalable and Customizable: Expand and customize by adding additional biomarkers using our PhenoCode 2.0 Molecular Barcoding Kit
Biomarkers Included in the Panel
The Human Metabolism Spike-In Module features key biomarkers covering glycolysis, oxidative phosphorylation, and amnio acid metabolism. The module has been tested and optimized for integration with the Human IO60 Discovery Panel, enabling visualization of how metabolic reprogramming shapes immune activation, nutrient competition, and redox balance within the TME. For integration into additional panels or incorporation of custom biomarkers, our Spatial Accelerator Laboratory Services team provides custom panel development and sample testing services to evaluate panel performance and deliver high-quality data for your specific research needs.
| Marker | Primary Function | Why it Matters for IO |
| ASCT2 |
Transports glutamine to fuel anabolic metabolism |
Reveals glutamine dependency in tumors and immune activation zones |
| ATP5A |
Drives mitochondrial ATP synthesis |
Indicates oxidative metabolism and mitochondrial activity |
| CS |
Initiates the TCA cycle; marker of mitochondrial fitness |
Highlights regions of high energy demand or metabolic integrity |
| G6PD |
Generates NADPH for antioxidant defense |
Identifies redox regulation and biosynthetic activity in proliferating cells |
| GLUT1 |
Imports glucose for glycolytic energy production |
Maps hypoxic, glycolytic niches within the tumor microenvironment |
| HK1 |
Catalyzes the first step of glycolysis |
Marks cells with high glycolytic flux and metabolic reprogramming |
| IDH2 |
Produces α-ketoglutarate and supports redox balance |
Links mitochondrial metabolism to epigenetic and redox control |
| LDHA |
Converts pyruvate to lactate under low oxygen |
Detects immunosuppressive, lactate-rich tumor regions |
| SDHA |
Links the TCA cycle to the electron transport chain |
Reflects mitochondrial respiration and metabolic competence |
Explore our database of validated antibodies to streamline adding additional biomarkers.
Key Research Applications:
Tumor immune microenvironment (TiME) metabolism profiling: Visualize how metabolic reprogramming shapes immune activation, nutrient competition, and redox balance within distinct spatial niches
Immunometabolism and therapy response: Evaluate how immune cells shift between glycolytic and oxidative programs in response to checkpoint blockade, CAR-T, or other immunotherapies
Hypoxia, oxidative stress, and metabolic rewiring: Investigate how cells adapt to low-oxygen or nutrient-stressed environments
Neuroinflammation biology: Investigate metabolic changes in neurons, astrocytes, and microglia in settings such as neurodegeneration, injury or inflammatory CNS disease
Patient stratification of clinical responses: Identify metabolic signatures in tumor and immune subsets will be crucial to elucidate the pathogenesis of the disease and reveal mechanistic insights underlying clinical response and resistance
Explore how ultrahigh-plex spatial phenotyping of cellular metabolism in the TiME revealed spatial metabolic signatures associated with immunotherapy sensitivity and resistance in cutaneous carcinoma.
The panel is available as:
Human Metabolism Spike-In Module
Purchase the 9-marker human metabolism module, which includes antibodies and reporters. The panel supports up to 25 slides at 1:200 dilution.
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Quanterix Spatial Accelerator Research Services
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