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Single Molecule Array (SiMoA™) technology is based upon the isolation of individual immunocomplexes on beads using standard ELISA reagents.
Beads with or without labeled immunocomplexes are loaded into arrays containing femtoliter-sized wells, and the arrays are sealed in the presence of the enzyme substrate and fluorescently imaged. Fluorescent product molecules of the enzyme-substrate reaction are confined in a 50 femtoliter volume, giving rise to a local high concentration of product within a matter of a few seconds that can be easily detected using a low cost imaging system.
By utilizing high density arrays containing 50,000-500,000 wells, hundreds to thousands of single immunocomplexes can be detected simultaneously. Isolation of single immunocomplexes in this way gives rise to a dramatic increase in sensitivity to enzyme labels over bulk, ensemble detection methods employed in traditional immunoassay technology. Quanterix has developed an instrument, reagents and optical fiber consumables for detecting a range of analytes in serum. SiMoA assays are easy to construct because they use the same biochemicals as traditional ELISA (antibodies, antigens and enzymes), but are routinely more than 1000-fold more sensitive than comparable ELISAs due to the single molecule nature of the technology.
 An image of the 3mm-sized fiber bundle with a higher magnification inset of uniform individual wells (left) and a fluorescent image of the optical fiber bundle (right). Illuminated spots represent single protein molecules that are detected and enumerated to determine analyte concentrations. |
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